Inhibitors of Protein Synthesis in Rat Liver Microsome Fractions
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منابع مشابه
Inhibitors of protein synthesis in rat liver microsome fractions.
Rat liver microsome fractions contain two general kinds of inhibitors of amino acid incorporation in vitro. The first is derived from lysosomes contaminating the microsome fractions. Lysosomes may be removed from rough vesicles (ribosomes plus endoplasmic membranes) by a density centrifugation technique. Lysosomal inhibition of incorporation in vitro is much more prominent in normal than in reg...
متن کاملStudies on the role of the morphological constituents of the microsome fraction from rat liver in protein synthesis.
It is established that the major protein-synthesizing activity of the liver cell resides in the microsome fraction. This has been shown both by studies in the intact animal and also by incubation of the isolated fraction. More recently evidence has been obtained for the synthesis of serum albumin by such a preparation (Campbell & Kernot, 1962; Sargent & Campbell, 1963). Morphologically the live...
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1. When the rat livers are perfused under the conditions of these experiments with rat blood diluted with saline, the livers remain capable of removing colloidal chromic phosphate normally for 4 hours or more; that is, the reticuloendothelial system continues to function normally. 2. Good rates of bile flow continue, generally for 4 hours. 3. The livers incorporate radioactivity from the amino ...
متن کاملBiotin and protein synthesis in rat liver.
Biotin administration to biotin-deficient rats enhanced protein synthesis. Time sequence studies indicated that RNA synthesis was stimulated as early as 1 hour after biotin administration to the deficient rat. The synthesis of RNA of liver was stimulated by biotin administration. Nuclear RNA isolated from biotin-injected rats was more active in a protein-synthesizing system in vitro than a simi...
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Alles, G. A. (1939). Amer. J. Physiol. 126, 420P. Blaschko, H. (1958). In 5-Hydroxytryptamine, pp. 50-57. Ed. by Lewis, G. P. London: Pergamon Press Ltd. Blaschko, H. & Himms, J. M. (1954). J. exp. Biol. 31, 1. Davison, A. N., Lessin, A. W. & Parkes, M. W. (1957). Experientia, 18, 329. Hope, D. B. (1959). Symp. biochem. Soc. 17, 93. Kobayashi, Y. (1957). Arch. Biochem. Biophy8. 71, 352. Kobayas...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96328-3